STFC-ISIS-14 Impact on buffer isotope content on protein interactions and structure.
04 Feb 2019







The stability of a protein is fundamental to its biological function, hence investigating the effect of solvation and hydrogen bonding on its structure and interactions is of great importance in biology and medicine. In this project we will investigate the structure and interactions of two globular proteins as a function of the solvent D2O content.

Benefits to the student: During this project the student will learn basic protein preparation and characterisation techniques (UV spectroscopy, UV-Vis; polyacrylamide gel electrophoresis, PAGE; small angle X-ray scattering, SAXS; dynamic and static light scattering, DLS&SLS) and analysis tools (molecular modelling of small angle scattering data). Two proteins will be studied at low concentration to determine their interaction potential (from SLS) and their solution structure (from SAXS). This will allow the student to learn the basics of protein biophysical characterisation. At the end of the project, the student will be able to determine the second virial coefficient and analyse the solution structure of model globular proteins. Finally the student will submit a scientific report summarising the main results obtained and the difficulties encountered.​

Supervisor: Najet Mahmoudi,